& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial   contains a 1 mL solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.   

Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

(-)-p-Bromolevamisole oxalate              373.2                      2.5 mM                      Alkaline phosphatases

Cantharidin                                            196.2                      500 µM                     Protein phosphatase 2A(PP2A)

Calyculin A                                            1009.2                    1 µM                         Protein phosphatases 1 and 2A(PP1 and PP2A)