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Perfect SYBR Green Master Mix, 2×
Perfect SYBR Green Master Mix, 2×
Price：
￥450.00~1750.00
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产品详情

货号

CV0001

品名

Perfect SYBR Green Master Mix, 2×

产品描述

Perfect SYBR Green Master Mix, 2× 是一种即用型的 2× 预混液，由 BesTaq HotStart 热启动酶、SYBR Green、dNTPs、Mg2+ 以及相关缓冲液组成，只需加入引物、模板和无菌水即可进行 qPCR 反应。本试剂盒仅用于科研用途，请勿用于临床诊断使用。

产品特点

特异性高：体系可防止非特异性扩增和引物二聚体

灵敏度好：可精确定量低至 10 拷贝的模板

线性范围广：可精确定量 9 个数量级

通用性好：几乎兼容所有的是荧光 PCR 仪

使用方便：实验过程无需冰上操作

保存条件

-20 ℃ 保存 12 个月，使用前充分溶解混匀

短期使用可放4℃ 保存，避免反复冻融

使用方法

1. 反应液配置

反应液成分

体积

终浓度

Perfect SYBR Green Master Mix

12.5 μL

1×

正向引物 （10 μM）

0.5 μL

200 nM

反向引物 （10 μM）

0.5 μL

200 nM

　　　　　　　     模板 *

                                                     5 μL

　　　　　　　　 总计

　　　　　　　　　　　　补 ddH2O 至 25 μL

*gDNA 的模板用量不超过 500ng，cDNA 不超过 50 ng

2. PCR 循环条件的设定

步骤

温度

时间

循环数

预变性

95℃

5 min

1 cycle

变性

95℃

10 s

40 cycles

退火、延伸

60℃

45 s

反应结束后应进行熔解曲线分析

3. 实验结果分析

根据扩增曲线和熔解曲线的结果进行荧光定量PCR数据分析

注意事项

1. SYBRGreen I 强光下易分解，使用时避免长时间强光照射本产品

2. 本制品不含内参ROX染料
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