Description

& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).

Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH₂O.

Storage

The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components

Ingredient                                                M.W.                         100x Conc.                      Target

Sodium Fluoride                                     42.0                           100 mM                          Acid phosphatases

Sodium Orthovanadate                          183.9                         100 mM                          Alkaline phosphatases, PTPs, ATPases

Sodium Tartrate Dihydrate                     230.1                         400 mM                          Acid phosphatases

Sodium Molybdate                                 205.9                         115 mM                          Acid and phosphoprotein phosphatases

Imidazole                                                68.1                           200 mM                         Alkaline phosphatases